Fluorescence is a process in which a molecule, quantum dot or other quantum emitter gets into an excited state by absorbing light and in return emits light of a different color. Because some energy is lost in the time the molecule is in the excited state, generally the emitted light is red-shifted with respect to the excitation wavelength. Fluorescence is used widely in biology to image the interior of organisms and spectroscopy can give valuable information on the physical conditions in the cell or allow for multiplexing
Ibsen’s transmission grating based spectrometers are ideally suited for fluorescence spectroscopy due to the following benefits:
